Coding

Part:BBa_K3544002:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)


Coding sequence of BcABA3, a member in biosynthetic gene cluster of ABA in B.cinerea


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 171
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 171
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 171
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 171
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An enzyme involved in the synthesis of ABA in Botrytis cinerea. It can catalyze Farnesyl diphosphate (FPP) into α-ionylideneethane.

Source

The original nucleotide sequence is from NCBI Botrytis cinerea B05.10 chromosome 8, NC_037317.1. The sequence is optimized according to the condon usage bias in Saccharomyces cerevisiae and synthesized from Sangon

References

Siewers V, Kokkelink L, Smedsgaard J, et al. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea[J]. Applied and Environmental Microbiology, 2006, 72(7): 4619-4626.