Translational_Unit

Part:BBa_K3526004:Design

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_city   (2020-10-18)


tac pro-atzR-T4 ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1026
    Illegal AgeI site found at 864
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to meet the type IIS standard, we made a synonymous mutation of Val243Val in the atzR sequence to remove BsaI restriction site.


Source

NCBI GenBank.

References