Composite

Part:BBa_K3523008:Design

Designed by: Kong Yangyang   Group: iGEM20_Xiamen_city   (2020-10-18)


J23116-rbs-Leader sequence-SOD-rbs-katA-Membrane Protein Ag43_700


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2166
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 2731
    Illegal AgeI site found at 2470
    Illegal AgeI site found at 2884
    Illegal AgeI site found at 3126
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To meet the type IIS standard, we made a synonymous mutation of the Glu56Glu in the coding sequence of SOD to remove the SapI restriction site and Ser283Ser in the coding sequence of catalase to remove the BsaI restriction site.



Source

GenBank: BAA02655.1 Catalase (EC:1.11.1.6)

References