T7

Part:BBa_K3521000:Design

Designed by: Kong Yangyang   Group: iGEM20_SHSID   (2020-10-13)


T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoters can produce high levels of transcription in E. coli when T7 RNAP is present. The second advantage of T7 promoters is that they are not recognized by E. coli RNAP making T7 promoters capable of independent regulation from E. coli promoters.


Source

Tabor, S. (1990), Expression Using the T7 RNA Polymerase/Promoter System. Current Protocols in Molecular Biology, 11: 16.2.1-16.2.11. doi:10.1002/0471142727.mb1602s11

References