Composite

Part:BBa_K3519012:Design

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)


araC-araBAD promoter-DNASEI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1043
    Illegal NheI site found at 1066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1388
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1205


Design Notes

The part is RFC 10 compatible.


Source

Chen CY, Lu SC, Liao TH. Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli: purification and characterization of the recombinant enzyme [published correction appears in Gene 1998 Jun 15;213(1-2):221]. Gene. 1998;206(2):181-184. doi:10.1016/s0378-1119(97)00582-9


References

Chen CY, Lu SC, Liao TH. Cloning, sequencing and expression of a cDNA encoding bovine pancreatic deoxyribonuclease I in Escherichia coli: purification and characterization of the recombinant enzyme [published correction appears in Gene 1998 Jun 15;213(1-2):221]. Gene. 1998;206(2):181-184. doi:10.1016/s0378-1119(97)00582-9