Composite

Part:BBa_K3519008

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)


medium promoter-traS

This part has the traS gene (BBa_K3519002) downstream to the medium strength Anderson promoter (BBa_J23118), the strong RBS (BBa_B0034) and is followed by the double terminator (BBa_B0015). Constitutive overexpression of the traS gene helps reduce DNA transfer between stable mating pairs.

Usage and Biology

Please refer to part BBa_K3519009 for more details on this part. The working of this gene is documented alongside BBa_K3519009 for better understanding.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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