Coding

Part:BBa_K3519002

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)


traS

This part codes for traS. The genes traS and traT help in surface exclusion and are carried naturally by the E. coli Fertiity factor (F factor). TraS is an inner membrane protein that prevents DNA transfer in stable mating pairs. The simultaneous overexpression of both these genes in E. coli has been shown to reduce DNA transfer by 33,000 times. Thus, they help reduce conjugation significantly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None