Coding

Part:BBa_K3519001

Designed by: Omkar Mohapatra   Group: iGEM20_IISER-Tirupati_India   (2020-10-07)


sulR

This part codes for sulR, flavin reductase. The genes sulX and sulR are a two-component flavin-dependent monooxygenase system present in Microbacterium sp. CJ77 and were first described by Kim et al., 2019. The simultaneous expression of both these genes in E. coli has been shown to degrade sulfonamides in the media.

Usage and Biology

Please refer to sulX (BBa_K3519000) for more details on this part. The working of this gene is documented alongside sulX for better understanding.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 410
    Illegal XhoI site found at 237
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None