Coding

Part:BBa_K3515012:Design

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-06-16)


Parathyroid Hormone Receptor with cysteine modification(s) to bind to a biosensor.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 26
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Isolated the N- terminal extracellular domain of PTHR, residues 1-60 for binding to PTH. Kept the one C48 residue in place to bind a corresponding cysteine linker arm (for biosensor immobilization). An intensive BLAST search was done to ensure that the C48 residue did not form a crucial interaction with the ligand.


Source

The source of this part is its sequence retrieved from the European Nucleotide Archive (V00303.1) along with our own modifications.

References

Nie, Y., Li, J., Liu, Y., Zhang, Q. and Ma, Q., 2020. A Visual FRET Immunofluorescent Biosensor for Ratiometric Parathyroid Hormone (1–84) Antigen Point-of-Care Detection. Journal of Fluorescence, pp.1-6.