Coding

Part:BBa_K3515005:Design

Designed by: Kody Klupt, Sono Khan   Group: iGEM20_Queens_Canada   (2020-05-22)


Glucose binding protein with cysteine modification(s) to bind to a biosensor.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added a cysteine for immobilization (D190C) opposite to the active site region for linker arm immobilization. Amino acid substitution considerations were made using an intensive BLAST search to ensure conservation.


Source

The source of this part is its sequence retrieved from the European Nucleotide Archive (M59444.1) along with our own modifications.

References

Fehr, M., Lalonde, S., Lager, I., Wolff, M.W. and Frommer, W.B., 2003. In vivo imaging of the dynamics of glucose uptake in the cytosol of COS-7 cells by fluorescent nanosensors. Journal of Biological Chemistry, 278(21), pp.19127-19133.