Part:BBa_K3515005
Glucose binding protein with cysteine modification(s) to bind to a biosensor.
Glucose/Galactose binding protein (GGBP) selectively binds glucose in its active site, inducing a conformation change in the N and C termini regions, respectively. This makes it a distinguishable candidate for in vivo or in vitro glucose monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with two fluorophores can permit glucose detection. Glucose detection is vital as glucose is used in cells to play an important role in critical metabolic pathways, which is crucial for maintaining energy homeostasis. Glucose is also a crucial biomarker used in clinical medicine for tracking the progression and status of patients with diabetes mellitus which may provide insights into pancreatic functioning and metabolic state. As such a biosensor for glucose tracking may be of great interest to patients and clinicians. This part includes a mutated GGBP to have a cysteine modification that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of glucose.
Cysteine immobilization modifications of binding protein. Protein structures were obtained from the RCSB Protein Data Bank. All protein residues are shown in a blue cartoon preset with cysteine residues labelled in red cartoon, respectively, using PyMOL software.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| biology | e coli |