Part:BBa_K3515004
Potassium binding protein with cysteine modification(s) to bind to a biosensor.
Potassium binding protein (Kbp) selectively binds potassium in its active site, inducing a conformation change in the N and C termini regions, respectively. This makes it a distinguishable candidate for in vivo or in vitro potassium monitoring using fluorescence resonance energy transfer (FRET). Coupling this protein with two fluorophores can permit potassium detection. Potassium detection is vital as potassium is used in cells to play an important role in fluid and electrolyte homeostasis, which is crucial for functioning of the neurological, cardiovascular, and immune system. Potassium is also a crucial biomarker used in clinical medicine for tracking the progression and status of patients with hypo- or hyperkalemia, which may provide insights into Chronic Kidney Disease (CKD) or diabetic ketoacidosis. As such a biosensor for potassium tracking may be of great interest to patients and clinicians. This part includes a mutated Kbp to have a cysteine modification that will bind cysteine linker arms and be used for biosensor immobilization allowing the detection of potassium.
Cysteine immobilization modifications of binding protein. Protein structures were obtained from the RCSB Protein Data Bank. All protein residues are shown in a blue cartoon preset with cysteine residues labelled in red cartoon, respectively, using PyMOL software.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 55
Illegal AgeI site found at 112
Illegal AgeI site found at 172 - 1000COMPATIBLE WITH RFC[1000]
| biology | e coli |