Part:BBa_K3512000
Sugarcane Invertase Inhibitor
This is the anti-invertase or invertase inhibitor from Sugarcane (Saccharum officinarum). It is part of the Fructose Regulated Anti-invertase Construct (BBa_K3512012).
In sugarcane, invertase enzymes play a key role in sucrose accumulation and are also involved in futile reactions where sucrose is continuously degraded during the pre- and post-harvest period, thereby reducing sugar yield and recovery. Invertase inhibitor (INVINH) proteins play a key role in post-translation regulation of plant invertases through which sucrose hydrolysis is controlled.
Homology Modelling
Homology modelling was used to create the structure of sugarcane invertase using SwissModel.
Ramachandran Plot
A Ramachandran plot is a way to visualize energetically favoured regions for backbone dihedral angles against amino acid residues in protein structure.
97.48% is Ramachandran Favoured. ALA120, PHE148 have C-Beta Deviations.
Quality Estimation
The comparison with Non-Redundant Set of PDB Structures helps us derive a quality estimate for our model via QMEAN4. The model had a GMQE of 1.24
Invertase Anti-invertase Docking
The following image illustrates the docking of sugarcane invertase with sugarcane invertase inhibitor. The final energy of binding is -667.5J. ClusPro docking server was used to perform the protein-protein docking.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 253
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 91
- 1000COMPATIBLE WITH RFC[1000]
| None |