Coding
Part:BBa_K351004:Design
Designed by: Dongchan Yang Group: iGEM10_KAIST-Korea (2010-07-22)
Modified Human FGF receptor for MPT51 antigen with fission yeast surface signal peptide
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 685
Illegal PstI site found at 175
Illegal PstI site found at 480
Illegal PstI site found at 1741 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1392
Illegal SpeI site found at 685
Illegal PstI site found at 175
Illegal PstI site found at 480
Illegal PstI site found at 1741 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2099
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 685
Illegal PstI site found at 175
Illegal PstI site found at 480
Illegal PstI site found at 1741 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 685
Illegal PstI site found at 175
Illegal PstI site found at 480
Illegal PstI site found at 1741
Illegal NgoMIV site found at 1119 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1401
Illegal BsaI.rc site found at 1889
Illegal BsaI.rc site found at 2253
Design Notes
This sequence contain signal peptide for fission yeast. You can't use this sequence directly for other organism like budding yeast.
Source
Signal petpdie is from Cell wall integrity and stress response component 1 of Fission yeast. Protein is from Normal human FGFR sequence without signal peptide. MPT51 binding domain is from "single chain antibody for Mycobacterium tuberculosis MPT51 antigen".