Coding

Part:BBa_K351001:Design

Designed by: Dongchan Yang   Group: iGEM10_KAIST-Korea   (2010-07-22)

Human STAT1 coding region


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
    Illegal NotI site found at 487
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 34
    Illegal EcoRI site found at 481
    Illegal SpeI site found at 475
    Illegal PstI site found at 218
    Illegal PstI site found at 495
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence don't contain promoter.


Source

Human STAT1(Isoform beta). from uniprot accession code P42224-2. This peptide is tblasn-ed to get coding region sequence.

References