Composite

Part:BBa_K3506030:Design

Designed by: Yiying Yu   Group: iGEM20_BNU-China   (2020-10-21)


The cassette of periodic expression and degradation of Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1578
    Illegal BglII site found at 2373
    Illegal BamHI site found at 2667
    Illegal XhoI site found at 203
    Illegal XhoI site found at 3173
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1496
    Illegal BsaI.rc site found at 2738
    Illegal BsaI.rc site found at 4094
    Illegal BsaI.rc site found at 4517
    Illegal BsaI.rc site found at 4529
    Illegal BsaI.rc site found at 5396
    Illegal SapI.rc site found at 4201
    Illegal SapI.rc site found at 4783
    Illegal SapI.rc site found at 4798


Design Notes

None.


Source

CLB2 promoter is from the genome of Saccharomyces cerevisiae BY4741.

Linker peptide KKKKS is synthesized de novo by GENEWIZ.

Cas9 is from plasmid pRH003.

References