Composite

Part:BBa_K3506004:Design

Designed by: Minglei Zhang   Group: iGEM20_BNU-China   (2020-10-13)


GFP coupled with Clb2（N 124aa）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1076
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

The function of GFP coupled with Clb2（N 124aa）is to characterize the periodic degradation function of Clb2（N 124aa） by the fluorescence generated from its expression when the gal1 is induced.


Source

This part derives from Saccharomyces cerevisiae BY4741.

References

[1]Hendrickson C , Iii M A M , Morabito L , et al. The KEN box regulates Clb2 proteolysis in G1 and at the metaphase-to-anaphase transition.[J]. Current Biology Cb, 2001, 11(22):1781-1787.