Composite
Part:BBa_K3506004
Designed by: Minglei Zhang Group: iGEM20_BNU-China (2020-10-13)
GFP coupled with Clb2(N 124aa)
GFP coupled with Clb2(N 124aa)is composed of gal1(),Clb2(N 124aa)() and GFP() through Linker().The purpose is to characterize the periodic degradation function of Clb2(N 124aa) by the fluorescence generated from its expression when the gal1 is induced.
Usage and Biology
This part was used to validate a modified label for protein degradation in yeast.It has a basic reference function for the design of other degradation tags and is beneficial to the research of protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1076
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
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Categories
Parameters
None |