Coding

Part:BBa_K3505041:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-23)


LacZa for Bacterial GoldenBraid plasmids


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 366
    Illegal PstI site found at 405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 366
    Illegal BamHI site found at 387
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 366
    Illegal XbaI site found at 393
    Illegal PstI site found at 405
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 616


Design Notes

The Judging form didn't let us to subbmit the whole plasmids for the Best Part Collection, because the whole plasmid had inside the restriction sites that made it not combatilbe for some Assembly. So we had to subbmit separatelly the backbone and the insert.