Coding
Part:BBa_K3505041
Designed by: Venetios Michelioudakis Group: iGEM20_Thessaly (2020-10-23)
LacZa for Bacterial GoldenBraid plasmids
This LacZa moudule is the cargo of the Bacterial GoldenBraid Plasmid Collection.
This LacZa module is flanked by the proper BsaI and BsmBI sites giving ID to the backbones
- pUPD2 backbone BBa_K3505043+ LacZa pUPD2 = BBa_K3505007
- alpha backbone BBa_K3505040+ LacZa α1R = BBa_K3505008
- alpha backbone BBa_K3505040+ LacZa α2 = BBa_K3505009
- omega backbone BBa_K3505042+ LacZa Ω1 = BBa_K3505010
- omega backbone BBa_K3505042+ LacZa Ω2 = BBa_K3505011
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 366
Illegal PstI site found at 405 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 366
Illegal BamHI site found at 387 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 366
Illegal XbaI site found at 393
Illegal PstI site found at 405 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 616
References
[1] Alejandro Sarrion-Perdigones, Marta Vazquez-Vilar, Jorge Palací, Bas Castelijns, Javier Forment, Peio Ziarsolo, José Blanca, Antonio Granell, Diego Orzaez (2013). “GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology.” Plant Physiology , 162 (3) 1618-1631; DOI: 10.1104/pp.113.217661
[edit]
Categories
Parameters
//awards/part_collection
//plasmid/construction
//plasmid/expression
//plasmid/construction
//plasmid/expression
None |