Composite
Part:BBa_K3505035:Design
Designed by: Venetios Michelioudakis Group: iGEM20_Thessaly (2020-10-20)
pAndersonJ23115:RBS-tyrosinase:AIDA-terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1502
Illegal EcoRI site found at 2198
Illegal XbaI site found at 1106
Illegal PstI site found at 1550
Illegal PstI site found at 2136 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1502
Illegal EcoRI site found at 2198
Illegal NheI site found at 11
Illegal NheI site found at 34
Illegal PstI site found at 1550
Illegal PstI site found at 2136
Illegal NotI site found at 1274 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1502
Illegal EcoRI site found at 2198
Illegal BglII site found at 534
Illegal BamHI site found at 517
Illegal BamHI site found at 2400 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1502
Illegal EcoRI site found at 2198
Illegal XbaI site found at 1106
Illegal PstI site found at 1550
Illegal PstI site found at 2136 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1502
Illegal EcoRI site found at 2198
Illegal XbaI site found at 1106
Illegal PstI site found at 1550
Illegal PstI site found at 2136
Illegal AgeI site found at 596 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This construct is cloned in a1RBBa_K3505008