Composite
Part:BBa_K3505027
Designed by: Venetios Michelioudakis, Magdalini Koroxenidou, Foteini Papadaki Group: iGEM20_Thessaly (2020-10-20)
PfliC:RBS-lac repressor:terminator
LacI BBa_K3505003uder control of a SCFAs inducible promoter pFLliC BBa_K2924016.
Usage and Biology
This Trancriscription Unit (TU) is activated form SCFAs and more specifically from Butyrate. Exressing the LacI protein for the inhibition of an other TU that is lac regulated.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in BBa_K3505008 and has overhangs compatible for GoldenBraid cloning.
Verification of cloning
Experimental Use and Experinece
This part is used in BBa_K3505036
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |