Part:BBa_K3505017

Double Terminator GB compatible with B6-C1 biology rrnBT1-T7TE chassis E.coli direction Forward forward_efficiency 0.984[CC] 0.97[JK] reversed_version 2146 reverse_efficiency 0.295[CC] 0.62[JK]

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B6-C1.

Figure 1.The overhangs of this part in the GoldenBraid Grammar.

Figure 2. The level 0 module : pupd2- double terminator. (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3

Source

Synthesized by IDT.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]