Coding

Part:BBa_K3503010:Design

Designed by: LEONG WENG I   Group: iGEM20_PuiChing_Macau   (2020-10-22)


CBD-alpha casein-RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1357
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1917
    Illegal AgeI site found at 2029
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE). RFP is added due to the adhesion test.

Source

The alpha-s1-casein is from the previous iGEM team, which was initiated based on Bos taurus;The cellulose-binding domain is from the previous iGEM team, which is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus.

References