Coding
Part:BBa_K3503001:Design
Designed by: LEONG WENG I Group: iGEM20_PuiChing_Macau (2020-10-19)
Alpha-s1-casein-His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 643
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
By adding 6*his, it can be used to detect the protein via anti-polyhistidine-tag antibodies or alternatively by in-gel staining (SDS-PAGE).
Source
The alpha-s1-casein is from the previous iGEM team, which was initiated based on Bos taurus;