Plasmid

Part:BBa_K3500009:Design

Designed by: Kennex Lam, Jiayi Lan   Group: iGEM20_GA_State_SW_Jiaotong   (2020-10-25)


pCB302-GUS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 542
    Illegal NgoMIV site found at 590
    Illegal NgoMIV site found at 722
    Illegal NgoMIV site found at 854
    Illegal NgoMIV site found at 1451
    Illegal NgoMIV site found at 1883
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 368
    Illegal BsaI.rc site found at 1403
    Illegal BsaI.rc site found at 1499
    Illegal BsaI.rc site found at 1787
    Illegal SapI.rc site found at 2083


Design Notes

The GFP + Terminator sequence originally on DinoIII was replaced as a whole by GUS + NOS terminator from pBI121.


Source

ten Lohuis, M., & Miller, D. . (1998). Genetic transformation of dinoflagellates (Amphidinium and Symbiodinium): expression of GUS in microalgae using heterologous promoter constructs. The Plant Journal, 13(3), 427-435. doi:10.1046/j.1365-313X.1998.00040.x

References