Coding
crtE-AB
Part:BBa_K349101:Experience
Designed by: David Lloyd Group: iGEM10_Alberta (2010-10-20)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K349101
Digestion at the BsaI cut sites inside of the prefix and suffix will create an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method.
User Reviews
UNIQ5b7fb783568865ad-partinfo-00000000-QINU Karina Arnesen- Alberta iGEM 2010 team- I've noticed that the B' end was designed in such a way that the stop codon is incorrectly placed, or non-existent when used with BA parts using the BioBytes v2.0 assembly method. UNIQ5b7fb783568865ad-partinfo-00000001-QINU