Coding
KanR-AB-Bs
Part:BBa_K349007:Experience
Designed by: David Lloyd Group: iGEM10_Alberta (2010-10-21)
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Applications of BBa_K349007
Digestion with BsaI around the recognition sites inside of the prefix and suffix will create a kan cassette with an A overhang at the 5' end and a B overhang at the 3' end. These ends are consistent with the BioBytes 2.0 assembly method. This is a base plasmid, meaning that the kan cassette could be digested out with BsaI and replaced with a PCR product that has had BsaI recognition sites and the appropriate AB ends incorporated into it. This allows for the production of more novel AB Bytes.
User Reviews
UNIQ8d5cc668443e3066-partinfo-00000000-QINU UNIQ8d5cc668443e3066-partinfo-00000001-QINU