Part:BBa_K349003:Design

RFP-BA-BbsI (for construction of BA BioBytes 2.0)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 793
  Illegal AgeI site found at 905
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part has built-in BbsI cutsites surrounding the J00450 RFP cassette. Digestion at these sites produces a B overhang at the 5' end and an A overhang at the 3' end consistent with the Biobytes 2.0 Assembly Method.

Source

pSB1C3