Coding

Part:BBa_K3487006

Designed by: Jianhua Zhou   Group: iGEM20_SZPT-CHINA   (2020-10-09)


Non-specific endonuclease

Description

BrnT as a non-specific endonuclease nucleic acid binding mRNA can affect the translation, leading to cell death can not express the normal protein.

pET28a(+), vector positive control ddH2O, negative control RalR, Non-specific endonuclease

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Heaton Brook E, Herrou Julien, Blackwell Anne E, et al. Molecular structure and function of the novel BrnT/BrnA toxin-antitoxin system of Brucella abortus.. 2012, 287(15):12098-110.

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Parameters
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