Reporter
sfGFP+ASV

Part:BBa_K3484006:Design

Designed by: Tomas Berjaga, Quim Marti & Jaume Puig   Group: iGEM20_UPF_Barcelona   (2020-10-25)


sfGFP + ASV tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
    Illegal AgeI site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of sfGFP was codon-optimized by Twist Bioscience in order to don't have sequencing errors, thus varying the nucleotide sequence from the original source (The amino acids sequence didn't change).


Source

Twist Bioscience

References