Composite
AHL sfGFP

Part:BBa_K3484004:Design

Designed by: Tomas Berjaga, Quim Marti & Jaume Puig   Group: iGEM20_UPF_Barcelona   (2020-10-26)


AHL sfGFP+ASV reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1249
    Illegal AgeI site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1142


Design Notes

The first step towards our lactone biosensor was to include the ASV degradation tag into the sfGFP sequence. This was done via a PCR, where one of the primers included an overhang which introduced this tag’s sequence at the end of the sfGFP coding region.

Once the tag had been added, the next step was the cloning of the lactone biosensor itself. Ths cloning was done using the Biobricks assembly.

Source

All the parts were provided by Synthetic Biology for Biomedical Applications laboratory in our univeristy, UPF. Except the sfGFP+ASV that was assembled.


References