Plasmid

Part:BBa_K3482024

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)


pLPT145 sponge plasmid(Potvin-Trottier, 2016)

This plasmid acts a molecular titration sponge for TetR, LacI and cI. It is intended to be used with a repressilator plasmid. It stabilizes the oscillations of the transcription factors of the repressilator.

Usage and Biology

The repressilator is a synthetic regulatory network of at least three genes in a plasmid that repress each other in a feedback loop. These genes are cyclically expressed one after the other in a loop at regular intervals over time. This property of cyclic expression of each gene allows us to produce our anti-cancer drug regularly for cancer treatment. For our system to work efficiently, the sponge plasmid is applied together with the repressilator to smooth out the observed oscillations by buffering excess proteins produced by the main repressilator.

All of the plasmids presented here were obtained and ordered from Addgene. They were all made available by the Professor Johan Paulson. All the plasmids come form this article Potvin-Trottier, L., Lord, N., Vinnicombe, G. et al. Synchronous long-term oscillations in a synthetic gene circuit. Nature 538, 514–517 (2016). https://doi.org/10.1038/nature19841.

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482023 .



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 75
    Illegal BglII site found at 2304
    Illegal XhoI site found at 369
    Illegal XhoI site found at 2485
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 75
    Illegal SpeI site found at 1342
    Illegal PstI site found at 108
    Illegal PstI site found at 2091
    Illegal NgoMIV site found at 1640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1580
    Illegal SapI site found at 1790


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Categories
Parameters
None