Plasmid

Part:BBa_K3482023:Design

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-23)


pLPT234 repressilator plasmid(Potvin-Trottier, 2016)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1956
    Illegal EcoRI site found at 2271
    Illegal EcoRI site found at 3042
    Illegal EcoRI site found at 3120
    Illegal EcoRI site found at 3289
    Illegal EcoRI site found at 4045
    Illegal EcoRI site found at 4314
    Illegal XbaI site found at 1197
    Illegal XbaI site found at 4980
    Illegal XbaI site found at 6311
    Illegal XbaI site found at 7276
    Illegal SpeI site found at 8952
    Illegal PstI site found at 3036
    Illegal PstI site found at 3283
    Illegal PstI site found at 5096
    Illegal PstI site found at 6427
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1956
    Illegal EcoRI site found at 2271
    Illegal EcoRI site found at 3042
    Illegal EcoRI site found at 3120
    Illegal EcoRI site found at 3289
    Illegal EcoRI site found at 4045
    Illegal EcoRI site found at 4314
    Illegal SpeI site found at 8952
    Illegal PstI site found at 3036
    Illegal PstI site found at 3283
    Illegal PstI site found at 5096
    Illegal PstI site found at 6427
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1956
    Illegal EcoRI site found at 2271
    Illegal EcoRI site found at 3042
    Illegal EcoRI site found at 3120
    Illegal EcoRI site found at 3289
    Illegal EcoRI site found at 4045
    Illegal EcoRI site found at 4314
    Illegal BamHI site found at 3024
    Illegal BamHI site found at 3271
    Illegal BamHI site found at 7282
    Illegal XhoI site found at 2036
    Illegal XhoI site found at 3074
    Illegal XhoI site found at 4125
    Illegal XhoI site found at 4153
    Illegal XhoI site found at 4234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1956
    Illegal EcoRI site found at 2271
    Illegal EcoRI site found at 3042
    Illegal EcoRI site found at 3120
    Illegal EcoRI site found at 3289
    Illegal EcoRI site found at 4045
    Illegal EcoRI site found at 4314
    Illegal XbaI site found at 1197
    Illegal XbaI site found at 4980
    Illegal XbaI site found at 6311
    Illegal XbaI site found at 7276
    Illegal SpeI site found at 8952
    Illegal PstI site found at 3036
    Illegal PstI site found at 3283
    Illegal PstI site found at 5096
    Illegal PstI site found at 6427
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1956
    Illegal EcoRI site found at 2271
    Illegal EcoRI site found at 3042
    Illegal EcoRI site found at 3120
    Illegal EcoRI site found at 3289
    Illegal EcoRI site found at 4045
    Illegal EcoRI site found at 4314
    Illegal XbaI site found at 1197
    Illegal XbaI site found at 4980
    Illegal XbaI site found at 6311
    Illegal XbaI site found at 7276
    Illegal SpeI site found at 8952
    Illegal PstI site found at 3036
    Illegal PstI site found at 3283
    Illegal PstI site found at 5096
    Illegal PstI site found at 6427
    Illegal NgoMIV site found at 7500
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1286
    Illegal BsaI site found at 3373
    Illegal BsaI.rc site found at 141
    Illegal BsaI.rc site found at 2756
    Illegal BsaI.rc site found at 2945


Design Notes

Temporary


Source

The plasmid originates from the following publication: doi: 10.1038/s41467-019-12638-z.

References