Composite

Part:BBa_K3482020

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)


aTc-inducible ccdA antitoxin

CcdA antitoxin under a pTet promoter

Usage and Biology

The CcdA protein gives immunity against the CcdB toxin by forming a strong complex CcdA-CcdB and therefore preventing the CcdB toxin to interact with the DNA gyrase.

This part is used to create a kill-switch in combination with the part BBa_K3482016 .

Characterization

All the data concerning the characterization of that part can be found in the composite part : BBa_K3482016 .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None