Coding

Part:BBa_K3482008

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)


Non-structural maintenance of chromosomes element 1 (Nse1)


Usage and Biology

Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. This part was used as a control for the 3xFLAG tag in western blot experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 37
    Illegal PstI site found at 172
    Illegal PstI site found at 388
    Illegal PstI site found at 430
    Illegal PstI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 37
    Illegal PstI site found at 172
    Illegal PstI site found at 388
    Illegal PstI site found at 430
    Illegal PstI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 37
    Illegal PstI site found at 172
    Illegal PstI site found at 388
    Illegal PstI site found at 430
    Illegal PstI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 37
    Illegal PstI site found at 172
    Illegal PstI site found at 388
    Illegal PstI site found at 430
    Illegal PstI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None