Tag

Part:BBa_K3482007

Designed by: Thierry Marti   Group: iGEM20_UNILausanne   (2020-10-19)


8x Histidine tag for protein purification

This 8x Histidine tag can be used for the affinity purification of a tagged protein.


Usage and Biology

The His tag has been used to purify the azurin secreted outside of the E. coli Nissle 1917 cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None