Translational_Unit

Part:BBa_K3477004:Design

Designed by: Mathilda Johansson   Group: iGEM20_Chalmers-Gothenburg   (2020-10-20)


Gene coding for his-tagged BphA2 enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 ter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 758
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal SpeI site found at 759
    Illegal PstI site found at 773
    Illegal NotI site found at 7
    Illegal NotI site found at 766
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 759
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 56
    Illegal SpeI site found at 759
    Illegal PstI site found at 773
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 456


Design Notes

-


Source

The genetic sequence comes from Pseudomonas sp. KKS102 and was found on Uniprot (https://www.uniprot.org/uniprot/P50206)


References