Translational_Unit

Part:BBa_K3477000:Design

Designed by: Mathilda Johansson   Group: iGEM20_Chalmers-Gothenburg   (2020-10-20)


Gene coding for his-tagged BphA1 enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 ter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1553
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal SpeI site found at 1554
    Illegal PstI site found at 1568
    Illegal NotI site found at 7
    Illegal NotI site found at 1561
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1554
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 56
    Illegal SpeI site found at 1554
    Illegal PstI site found at 1568
    Illegal NgoMIV site found at 1215
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The promoter, RBS, terminator and biobrick prefix/suffix come from the iGEM parts registry. The genetic sequence originates from Pseudomonas sp. KKS102 and was found on Uniprot (https://www.uniprot.org/uniprot/Q52438)

References