DNA

Part:BBa_K3476013:Design

Designed by: Daria Shumkova   Group: iGEM20_MSP-Maastricht   (2020-10-19)


shRNA EF1A_a (Thaumetopoea processionea)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 54
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Loop sequence: CAAGA and 5`UU-overhang


Source

Thaumetopoea processionea partial EF-1a gene for elongation factor-1 alpha GenBank: HE861906.1

References