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DNA
Part:BBa_K3476013:Design
Designed by: Daria Shumkova Group: iGEM20_MSP-Maastricht (2020-10-19)
shRNA EF1A_a (Thaumetopoea processionea)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BamHI site found at 54 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Loop sequence: CAAGA and 5`UU-overhang
Source
Thaumetopoea processionea partial EF-1a gene for elongation factor-1 alpha GenBank: HE861906.1