T7

Part:BBa_K3476011:Design

Designed by: Silvio Salvatore Bonni   Group: iGEM20_MSP-Maastricht   (2020-10-19)


bacterial shRNA_expression_cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 56
    Illegal NheI site found at 116
    Illegal NotI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 56
    Illegal BamHI site found at 62
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 56
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added T7 terminator from T444T for effective transcription termination.


Source

Plasmids: L4440; T444T

References