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DNA
Part:BBa_K3476006:Design
Designed by: Marta Rubina Group: iGEM20_MSP-Maastricht (2020-10-17)
shRNA Tha p 2_a (Thaumetopoea processionea)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BamHI site found at 52 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 23
Design Notes
Restriction sites for EcoR1 & BamH1 Hairpin loop
Source
Thaumetopoea processionea Tha p 2 gene for Allergenic protein Tha p 2, clone 2 (LM994939.1)