DNA

Part:BBa_K3476006:Design

Designed by: Marta Rubina   Group: iGEM20_MSP-Maastricht   (2020-10-17)


shRNA Tha p 2_a (Thaumetopoea processionea)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 52
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 23


Design Notes

Restriction sites for EcoR1 & BamH1 Hairpin loop



Source

Thaumetopoea processionea Tha p 2 gene for Allergenic protein Tha p 2, clone 2 (LM994939.1)


References