DNA

Part:BBa_K3476000:Design

Designed by: Silvio Salvatore Bonni   Group: iGEM20_MSP-Maastricht   (2020-10-16)


shRNA Wg_a (Thaumetopoea processionea)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 54
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Loop sequence: CAAGA 5`UU-overhang


Source

Thaumetopoea processionea partial wg gene for wingless GenBank: HE861920.1 https://www.ncbi.nlm.nih.gov/nuccore/HE861920.1

References