Coding

Part:BBa_K3468071

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)


PETase A47S

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A to S at 47 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Add hydrogen bonds. According to the prediction of FoldX, DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into SER, it formed hydrogen bonds with GLY-48 and SER-103, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.

Fig.1 Ala47 in PyMOL
Fig.2 A47S in PyMOL


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Parameters
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