Coding

Part:BBa_K3468069

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)


PETase R90D

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from R to D at 90 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds stabilized α3 helix and β3-α2loop According to the prediction by FoldX, DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after it was mutated into ASP, it formed hydrogen bonds with 92-SER and 93-SER, which stabilized α3 helix and β3-α2loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 R90D in PyMOL


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