Coding

Part:BBa_K3468044

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase S136N

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from S to N at 136 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds stabilized α3-β5loop According to the prediction of FoldX, DDG decreased, and PyOML was used for visual screening, which formed hydrogen bonds with 132-ARG, 139-GLY and 140-THR. after mutating it into ASN, the number of hydrogen bonds increased, which stabilized α3-β5loop and was beneficial to improve the thermal stability of PETase.

Fig.1 S136N in PyMOL
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Categories
Parameters
None