Coding

Part:BBa_K3468040

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase K148R

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from K to R at 148 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Adding hydrogen bonds stabilized α3-β5loop, β2-β3loop and β2sheet

It was predicted by FoldX that DDG decreased. PyOML was used for visual screening, which formed hydrogen bonds with 78-VAL, 144-Pro and 145-ILE. after mutation to ARG, it formed new hydrogen bonds with 71-PRO and 72-THR, which stabilized α3-β5loop, β2-β3loop and β2sheet, 

which was beneficial to improve the thermal stability of PETase
Fig.1 K148R in PyMOL


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