Coding

Part:BBa_K3468037

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase A152N

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A to N at 152 position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Applications of BBa_K3468037

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 Ala152 in PyMOL
Fig.2 A152N in PyMOL

Adding hydrogen bonds stabilized α3-β5loop and α4-β6loop


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