Coding

Part:BBa_K3468034:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)


PETase T116R


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We originally planned to increase the number of salt bridges through sequence alignment of homologous enzyme Cut190. Replace uncharged amino acids with charged amino acids to form electrostatic interactions with surrounding amino acids.


Source

Ideonalla sakaiensis

References