Part:BBa_K3467009:Experience
This year, we attained part from team CAU_China 2019 and improved its part INP-CEN (BBa_K3279008). By using RT-PCR, we extracted AGA2 cDNA from yeast
In order to enhance yeast ability on fermentation, especially in cellulose degradation so that farmer could reuse the yeast powder to get more yeast biopesticide. We employed yeast surface performance protein AGA2. The endoglucanase fused with AGA2 protein can anchor in the yeast cell wall. We’ve already the part and inserted it into our plasmid. After we transferred AGA2-CEN part into our engineering yeast, we’ll test its endoglucanase enzyme activity. In addition, we fused a 6His-tag on the C-terminal of endoglucanase so that we can use immunofluorescence staining to identify the position of CEN protein.